Abcam, ab77643, Anti-NPEPPS antibody

Size: 200µL
Goat Polyclonal NPEPPS antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NPEPPS aa 850 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NPEPPS aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P55786

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NPEPPS also known as aminopeptidase puromycin sensitive or APP is a metalloprotease with an approximate molecular mass of 103 kDa. It functions by catalyzing the cleavage of N-terminal amino acids from peptides which is important for many cellular processes. NPEPPS localizes to both the cytoplasm and nucleus within cells and it shows widespread expression across various tissues especially in the brain heart and liver. Its activity plays an important role in the breakdown and recycling of intracellular proteins.
Biological function summary
NPEPPS influences protein turnover by participating in peptide degradation and processing. The enzyme associates with complexes involved in antigen processing impacting the production of antigenic peptides presented by MHC class I molecules. By regulating peptide availability NPEPPS facilitates immune responses and maintains cellular protein homeostasis. This regulatory effect makes it important for several physiological and cellular functions emphasizing its involvement beyond simple peptide cleavage.
Pathways
The NPEPPS enzyme influences the ubiquitin-proteasome system an essential cellular mechanism for protein degradation. This pathway requires NPEPPS for the final trimming of peptides before they are presented by the immune system. The enzyme's activity also connects to the mTOR signaling pathway which controls cell growth and metabolism. Proteins such as ubiquitin and components of the proteasome interact with NPEPPS linking its mechanical function to larger cellular and metabolic pathways.
NPEPPS has associations with neurodegenerative diseases like Alzheimer's disease and Huntington's disease. Altered enzyme function can affect peptide accumulation potentially contributing to the pathological protein aggregates observed in these diseases. Investigations also show a connection to proteins such as amyloid precursor protein implicating NPEPPS in pathways leading to amyloid plaque formation in Alzheimer's. These interactions highlight the potential impact of NPEPPS in disease mechanisms and as a target for therapeutic intervention.