Abcam, ab77779, Anti-DIO2 antibody

Size: 100µg
Rabbit Polyclonal DIO2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 12 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
'DIO2' also known as Type II iodothyronine deiodinase is an enzyme responsible for the conversion of thyroxine (T4) to the active hormone triiodothyronine (T3) by removal of an iodine atom. This transformation is important for regulating thyroid hormone activity. DIO2 has a molecular mass of approximately 58 kDa and is expressed predominantly in tissues such as the brain pituitary gland brown adipose tissue and skeletal muscle. It plays an integral role in maintaining intracellular T3 levels necessary for various physiological functions.
Biological function summary
DIO2 modulates local T3 availability which impacts metabolic rate thermogenesis and development. DIO2 is not part of a protein complex but interacts closely with thyroid hormone receptors that are activated by the produced T3. In adipocytes and myocytes DIO2-mediated T3 generation ensures the hormones' optimal action affecting processes such as energy expenditure and muscle function. This activity is critical in adapting to changing metabolic demands.
Pathways
DIO2 is integral to the thyroid hormone signaling pathway significantly influencing processes governed by thyroid hormone function. By converting T4 to T3 DIO2 interacts with thyroid hormone receptors and influences downstream effectors linked to metabolism and growth. DIO2 activity is regulated via the hypothalamic-pituitary-thyroid axis ensuring proper feedback loops and homeostasis. Additionally it is related to other deiodinases like DIO1 and DIO3 which also participate in thyroid hormone modification.
Alterations in DIO2 expression or activity have been linked to thyroid-related conditions such as hypothyroidism and Graves' disease. In hypothyroidism decreased DIO2 activity could lead to reduced local T3 levels affecting cellular metabolism. In Graves' disease overactive DIO2 may result in excessive T3 production exacerbating symptoms. DIO2's action further connects with thyroid hormone receptor beta (THRB) where abnormalities can contribute to resistance to thyroid hormones influencing disease progression and treatment outcomes.