Abcam, ab79107, APC/Cy7® Anti-CD45R antibody [RA3-6B2]

Size: 50µg
Rat Monoclonal CD45 antibody - conjugated to APC/Cy7®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:RA3-6B2,
Isotype:IgG2a,
Light chain type:kappa,
Conjugation:APC/Cy7®,
Excitation/Emission:Ex: 650nm, Em: 785nm,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from TCS, Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD45R also known as Leukocyte Common Antigen or LCA is a protein that plays a significant role in immune cell function. It is an isoform of the larger protein CD45 which includes several alternatively spliced variants. CD45R is a transmembrane protein tyrosine phosphatase with a mass ranging from 180 to 220 kDa depending on its isoform. This protein is primarily expressed on hematopoietic cells including T cells B cells and other leukocytes. It functions mechanically by modulating signaling thresholds essential for lymphocyte development and activation.
Biological function summary
CD45R influences signaling pathways critical for immune response regulation. It does not form a complex by itself but interacts with other membrane receptors and cytoplasmic kinases to mediate signal transduction. The protein adjusts the activity of Src family kinases through its phosphatase activity impacting cell-cell communication and responsive behavior to antigens. CD45R is involved in maintaining a functional immune surveillance system particularly ensuring the adequate responsiveness of lymphocytes.
Pathways
CD45R's phosphatase activity is integral to the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways. This activity regulates Lck and Fyn kinases within these pathways contributing to proper lymphocyte activation and development. Any disruption in CD45R function can affect these pathways potentially impacting proteins like ZAP-70 and Syk which are essential for signal transduction downstream of the TCR and BCR respectively.
CD45R has been implicated in conditions such as severe combined immunodeficiency (SCID) and autoimmune diseases like multiple sclerosis. Its malfunction or aberrant expression can cause improper immune system activation or suppression. In autoimmune contexts CD45R's interaction with other proteins like CTLA-4 may become dysregulated leading to immune system attacks on the body's tissues. Understanding these connections helps in developing therapeutic strategies targeting immune dysfunctions.