Abcam, ab79157, Anti-VGLUT2 antibody [8G9.2]

Size: 50µg
Anti-VGLUT2 antibody [8G9.2] (ab79157) is a mouse monoclonal antibody detecting VGLUT2 in Western Blot, IHC-FrFL . Suitable for Mouse, Rat . - Over 40 publications - Trusted since 2009
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:8G9.2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:WB, IHC-FrFlSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Rat Slc17a6.Q9JI12,
Specificity:Some customers have successfully used ab79157 with IHC-Fr and IHC-P. We recommend ab216463 as an alternative for IHC-P and IHC-Fr. For eliminating background when staining mouse tissue with this mouse monoclonal antibody, please refer to the Mouse-on-mouse (MOM) staining protocol.

Product details:
What is this antibody validated in?
Anti-VGLUT2 antibody [8G9.2] (ab79157) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.
What is the molecular weight of VGLUT2?
Anti-VGLUT2 [8G9.2] (ab79157) specifically detects a band for VGLUT2 (UniProt: Q9JI12) at a molecular weight of 64kDa.
Trusted by the scientific community
Anti-VGLUT2 [8G9.2] (ab79157) was first used in a scientific publication in 2009 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-VGLUT2 [8G9.2] (ab79157) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.6Preservative: 0.1% Sodium azideConstituents: 1.45% Sodium chloride, 0.328% Sodium phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VGLUT2 also known as Vesicular Glutamate Transporter 2 or SLC17A6 plays a significant role in neurotransmission. This protein facilitates the transport of glutamate an excitatory neurotransmitter into synaptic vesicles in preparation for synaptic release. VGLUT2 has a molecular weight of approximately 61 kDa. Its expression appears particularly in the central nervous system including regions like the thalamus brainstem and some parts of the cerebral cortex.
Biological function summary
VGLUT2 is essential for proper glutamatergic signaling in neuronal communication. The protein does not form part of a larger complex but functions individually to maintain synaptic vesicle filling with glutamate. Normal glutamatergic synaptic transmission relies on VGLUT2’s ability to concentrate glutamate in synaptic vesicles. This concentration is necessary for effective excitatory signaling impacting processes such as synaptic plasticity learning and memory.
Pathways
VGLUT2 serves a critical function in neurotransmitter release and synaptic vesicle cycle. The protein actively transports glutamate into synaptic vesicles enabling its release into the synaptic cleft. VGLUT2 interacts closely with proteins like SNAREs that assist in vesicle docking and fusion. The protein also plays a functional role in the glutamatergic pathway which is important for excitatory neurotransmission in the brain.
VGLUT2 holds relevance to conditions such as schizophrenia and epilepsy. Its dysregulation can alter glutamate levels potentially contributing to altered neuronal excitability and synaptic dysfunction. Studies link VGLUT2 with connected proteins like NMDA receptors which mediate excitatory postsynaptic potentials and are associated with glutamatergic signaling disruptions in these disorders. Alterations in the expression or function of VGLUT2 might influence susceptibility to these neuropsychiatric and neurological conditions.