Abcam, ab79199, Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody

Size: 100µg
Rabbit Polyclonal STK3/MST-2 phospho T180 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human MST1 phospho T183.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MST1 phospho T183. The exact immunogen used to generate this antibody is proprietary information.P26927,
Specificity:ab79199 detects endogenous levels of STK3/MST1 only when phosphorylated at threonine 183 (MST1) and/or threonine T180 (STK3).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab79199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
STK3 also known as MST-2 and MSP also referred to as MST1 are serine/threonine kinases belonging to the Ste20 family. MST1 has a molecular weight of approximately 56 kDa while MST2 has a molecular weight of about 58 kDa. These proteins are expressed in various tissues with notable presence in the liver heart and brain. They function mechanistically through phosphorylation of downstream effectors and serve as core components of the Hippo signaling pathway which regulates cell proliferation apoptosis and organ size.
Biological function summary
These kinases play essential roles in regulating cell growth and apoptosis. They often exist as a part of larger complexes that include other signaling components and proteins. STK3 and MSP interact with a network of proteins including tumor suppressors like LATS1/2 to mediate their biological activities. These interactions allow for proper cellular response to environmental signals and maintain cellular homeostasis.
Pathways
STK3 and MSP hold significant importance in the Hippo signaling pathway and the oxidative stress response pathway. In the Hippo pathway they phosphorylate and activate downstream effectors such as YAP and TAZ which ultimately regulate gene expression related to cell proliferation and apoptosis. In the oxidative stress response these kinases interact with FOXO proteins to modulate the cellular response to oxidative damage linking them to cellular aging and longevity processes.
Aberrations in STK3 and MSP functions link to cancer and neurodegenerative diseases. In cancer dysfunction in the Hippo pathway can result in uncontrolled cell growth with proteins like YAP and TAZ playing pivotal roles in tumorigenesis. In neurodegenerative diseases changes in MSP function or expression can contribute to altered neuronal survival and apoptosis which implicates proteins like FOXO in regulating neuronal responses and stress resistance.