Abcam, ab80, Anti-CDKN2A/p19ARF antibody

Size: 100µg
Anti-CDKN2A/p19ARF antibody (ab80) is a rabbit polyclonal antibody detecting CDKN2A/p19ARF in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Mouse . - Over 220 publications - Trusted since 2002
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC, ICC/IF, IHC-Fr, IHC-P, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:On Western blot the antibody reveals IVT murine p19ARF and a band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs.Replenishment batches of ab80 are tested in WB. Previous batches were additionally validated in ELISA, ICC, ICC/IF, IHC-Fr and IHC-P. These applications are still expected to work and are covered by our Abcam product promise.

Product details:
What is this antibody validated in?
Anti-CDKN2A/p19ARF antibody (ab80) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Mouse samples.
What is the molecular weight of CDKN2A/p19ARF?
Anti-CDKN2A/p19ARF (ab80) specifically detects a band for CDKN2A/p19ARF (UniProt: Q64364) at a molecular weight of 17kDa.
Trusted by the scientific community
Anti-CDKN2A/p19ARF (ab80) was first used in a scientific publication in 2002 and has been cited over 220 times in peer-reviewed journals.
Reviewed by scientists
Anti-CDKN2A/p19ARF (ab80) has over 15 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The serum has been purified on a peptide-NHS-activated Sepharose column. Specific antibodies have been eluted with 100 mM glycine pH 2.5. The purified antibodies have been dialysed against PBS pH 7.4., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle