Abcam, ab80033, Recombinant human Hsp90 beta protein (Active)

Size: 50µg / 100µg
Recombinant human Hsp90 beta protein (Active) is a Human Full Length protein, in the 1 to 724 aa range, expressed in Baculovirus infected BTI-TN-5B1-4, with >90% purity and suitable for western blot, SDS-PAGE, ELISA, Functional studies and more.The predicted molecular weight of ab80033 protein is 90 kDa. - Save time and ensure accurate results - use our Hsp90 beta protein as a control - Optimal protein bioactivity, stability and reproducibility - Available in different sizes to fit your experimental needs
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected BTI-TN-5B1-4 cells,
Tags:Tag free,
Applications:ELISA, WB, IP, SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:ATPase active,
Accession:P08238,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 10% Glycerol (glycerin, glycerine), 1.015% Sodium chloride, 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA

Product details:
Ensure the validity of your result using our bioactive recombinant human Hsp90 beta protein ab80033 as a positive control in SDS-PAGE and western blot. ab80033 is endotoxin free. <0.1 EU/mL.
Analyze your Hsp90 beta ELISA data using the ab80033 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE
Check out of western blot protocol for more information
Check out our ELISA protocol for more information

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hsp90 beta also known as Hsp90AB1 or Hsp90 protein is a heat shock protein of approximately 90 kDa. It is a molecular chaperone found in most eukaryotic cells. Hsp90 beta helps in the proper folding stabilization and degradation of many proteins. Unlike its isoform Hsp90 alpha Hsp90 beta has a more stable expression and is not typically induced by stress. This protein is primarily localized in the cytosol but can also be present in other cellular compartments depending on the cellular state.
Biological function summary
Hsp90 beta functions to maintain protein homeostasis and cellular integrity. It forms part of a multi-protein chaperone complex which includes cochaperones such as Hop Hsp70 and p23 necessary for its full functionality. Hsp90 beta supports the maturation of steroid hormone receptors kinases and other client proteins. It plays an important role in the cell cycle regulation through its interaction with various proteins ensuring proper cell division and growth.
Pathways
Hsp90 beta is deeply involved in signal transduction and cellular stress response pathways. Its interaction with the Akt pathway is significant for cell survival signals. Hsp90 beta also participates in the MAP kinase pathway affecting cell growth and differentiation. The protein associates with multiple kinases including RAF and Src which are important for downstream signaling.
The dysregulation of Hsp90 beta is associated with cancer and neurodegenerative diseases. In cancer Hsp90 beta stabilizes many oncoproteins making it a potential therapeutic target for inhibiting tumor growth. It interacts with client proteins like the proto-oncogene c-Src promoting tumorigenesis. In neurodegenerative disorders such as Alzheimer's disease improper interaction between Hsp90 beta and tau proteins can contribute to disease progression impacting neuronal function.