Abcam, ab80049, Anti-SMAD6 antibody

Size: 200µL
Rabbit Polyclonal SMAD6 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD6.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SMAD6. The exact immunogen used to generate this antibody is proprietary information.O43541,
Specificity:This reacts specifically with human 54 kDa SMAD6 isoform D protein. It is not expected to cross react with other species.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMAD6 also known as MADH6 or SMAD family member 6 is an inhibitory regulator in the TGF-beta signaling pathway. It has a molecular mass of approximately 53 kDa. SMAD6 specifically binds to and inhibits the BMP (Bone Morphogenetic Protein) receptor-activated SMAD proteins mainly SMAD1 SMAD5 and SMAD8 preventing their phosphorylation and subsequent translocation into the nucleus. This protein is expressed in various tissues including the heart skeletal muscle and brain.
Biological function summary
SMAD6 plays a critical role in modulating BMP signaling. It functions not as part of a larger protein complex but as a direct antagonist to BMP-induced cellular responses. By binding to SMAD1 and SMAD5 SMAD6 impedes the transcriptional activity that promotes various cellular processes such as proliferation differentiation and apoptosis. This regulation is essential for maintaining cellular homeostasis and ensuring proper embryonic development.
Pathways
SMAD6 serves as an important checkpoint within the TGF-beta/BMP signaling pathway. It relates to other proteins such as SMAD7 which also functions as an inhibitory SMAD although SMAD6 is more selective in antagonizing BMP receptors. Through its actions SMAD6 aids in the regulation of processes like bone formation and vascular development. By throttling the BMP pathway SMAD6 maintains a balance between proliferative signals and inhibitory cues important for organogenesis.
Disrupted SMAD6 function associates with cardiovascular and skeletal abnormalities. Mutations or altered expression levels in SMAD6 have links to congenital heart defects where it fails to adequately regulate BMP signaling. This dysregulation can also involve proteins such as SMAD1 leading to improper cardiac development. Additionally SMAD6 might affect conditions like fibrodysplasia ossificans progressiva that feature abnormal bone growth further implicating the need for balanced BMP signaling in maintaining tissue integrity.