Abcam, ab80495, Anti-15 Lipoxygenase 1 antibody

Size: 100µg
Goat Polyclonal 15 Lipoxygenase 1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ALOX15 aa 450-500.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ALOX15 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.P16050

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab80495 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.5% BSA, 0.5% Tris buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
15-Lipoxygenase 1 also known as 15-LOX-1 is an enzyme with significant mechanical roles in the human body. Its main function is to catalyze the oxidation of fatty acids especially converting arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HPETE). The protein has other names like arachidonate 15-lipoxygenase or 15-LO. The molecular weight of 15-LOX-1 is approximately 75 kDa. It is mainly expressed in cells of the reticulocyte and tracheobronchial epithelium as well as in macrophages and eosinophils.
Biological function summary
15-Lipoxygenase 1 plays a role in the immune and inflammatory responses. It is involved in lipid mediator biosynthesis producing reactive lipids that influence cell signaling and modulate reactions to inflammation. 15-LOX-1 acts as part of a complex with other enzymes within the lipoxygenase family which includes 5-lipoxygenase and 12-lipoxygenase. This enzyme can also be important for cell maturity and has roles in cell differentiation and maturation particularly in inflamed tissues.
Pathways
Several significant processes involve 15-Lipoxygenase 1 especially its part in the arachidonic acid metabolism and the linoleic acid pathway. In these pathways 15-LOX-1 transforms substances into various lipid signaling molecules like lipoxins and resolvins. These molecules have connections with other proteins like cyclooxygenase-2 (COX-2) and 5-LO which participate in the synthesis of other eicosanoids. The interplay of these pathways and proteins helps in regulating inflammation and resolving it ensuring a balanced biological response to stimuli.
15-Lipoxygenase 1 has associations with conditions including asthma and atherosclerosis. In asthma the enzyme contributes to the inflammatory changes observed in affected airways. Its involvement in this respiratory disorder links it with proteins such as LTC4 synthase that generate pro-inflammatory leukotrienes. In atherosclerosis 15-LOX-1 interacts with oxidized low-density lipoprotein (LDL) in arterial walls which contributes to plaque formation and the associated chronic inflammation. These roles reveal the enzyme's significance in both chronic inflammation and cardiovascular disease settings.