Abcam, ab80600, Anti-Smg1 antibody

Size: 100µg
Rabbit Polyclonal SMG1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SMG1 aa 1250-1300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SMG1 aa 1250-1300. The exact immunogen used to generate this antibody is proprietary information.Q96Q15

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Smg1 also known as SMG-1 is a large protein kinase with a molecular mass of approximately 410 kDa. It localizes mainly in the cytoplasm and nucleus of cells and has an important mechanical role in phosphorylating proteins to regulate various cellular processes. As a part of the phosphatidylinositol 3-kinase-related kinase (PIKK) family Smg1 exhibits enzymatic activity that is important for maintaining cellular functions.
Biological function summary
Smg1 plays an important part in the nonsense-mediated mRNA decay (NMD) pathway an important cellular process for RNA surveillance mechanisms. Smg1 associates with other proteins forming a complex known as the SMG1C complex which monitors and degrades faulty mRNAs that contain premature stop codons to prevent the production of truncated proteins. Smg1 aids in safeguarding the cellular transcriptome integrity by ensuring quality control at the mRNA level.
Pathways
Smg1 interacts importantly within the NMD pathway alongside proteins UPF1 and UPF2 which are essential for proper function. Smg1 is also involved in the cellular response to stress pathways contributing to the regulation of stress granules. Through these pathways Smg1 ensures proper adaptation and response to changing cellular conditions maintaining overall cell health and functionality.
Smg1 has implications in cancer and genetic disorders. Aberrations in Smg1 activity or expression can lead to improper mRNA decay contributing to tumorigenesis by allowing defective mRNAs to produce malfunctioning proteins. In cancer Smg1 regulates p53 a protein that is associated with cell cycle control and apoptosis. Additionally mutations affecting Smg1 can relate to neurodevelopmental disorders where it influences proteins involved in mRNA processing pathways highlighting its role in nervous system function and development.