Abcam, ab8086, Anti-Endothelium antibody [PAL-E]

Size: 1mL
Mouse Monoclonal Endothelium antibody. Suitable for IHC-Fr and reacts with Human, Pig, Dog, Goat, Rabbit samples. Cited in 12 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:PAL-E,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Rabbit, Goat, Dog, Human, Pig,
Applications:IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Reactivity with: Lymphatic endothelium :not detectable. Sinus histiocytes :not detectable. Megakaryocytes :not detectable.

Product details:
The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.3Preservative: 0.07% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The endothelium a monolayer of cells lining blood vessels plays an important role in vascular function and maintenance. Endothelial cells have a mass of approximately 35 kDa and are found in arteries veins and capillaries including the heart and lymphatic vessels. These cells form a selective barrier between the bloodstream and surrounding tissues regulating the exchange of fluids solutes and macromolecules. The endothelium also modulates blood flow and pressure through interaction with vascular smooth muscle cells.
Biological function summary
The endothelium contributes to several critical processes including vascular homeostasis coagulation and inflammation regulation. It operates as part of the vascular system interacting with components like platelets and leukocytes to mediate immune responses. The endothelium produces signaling molecules such as nitric oxide (NO) which is essential for vasodilation. This layer also responds to mechanical stimuli from blood flow adapting its structure and function accordingly.
Pathways
The endothelium engages in the nitric oxide and endothelin pathways which balance vasodilation and vasoconstriction. In the nitric oxide pathway the enzyme endothelial nitric oxide synthase (eNOS) becomes activated producing NO that signals smooth muscle relaxation. Within the endothelin pathway endothelin-1 interacts with vascular smooth muscle cells causing vasoconstriction. Both pathways are integral to maintaining vascular tone and regulating blood pressure.
The endothelium's dysfunction is associated with conditions such as atherosclerosis and hypertension. In atherosclerosis the impaired endothelial function leads to plaque formation and arterial stiffness. Hypertension involves chronic constriction and reduced NO bioavailability affecting endothelium-mediated vasodilation. Related proteins include low-density lipoprotein (LDL) for atherosclerosis and angiotensin-converting enzyme (ACE) in hypertension both impacting endothelial health and function.