Abcam, ab81224, Anti-PABPN1 antibody [EP3001Y]

Size: 100µL
Rabbit Monoclonal PABPN1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP3001Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PABPN1 also known as polyadenylate-binding nuclear protein 1 is an RNA-binding protein that plays a fundamental role in the regulation of mRNA processing. It is a 31-kilodalton protein that binds to the poly(A) tail of pre-mRNA and stimulates the assembly of the polyadenylation complex thereby influencing the maturation and stability of mRNA transcripts. This protein is predominantly expressed in the nucleus of eukaryotic cells where it performs its important function in post-transcriptional regulation.
Biological function summary
PABPN1 is involved in controlling the polyadenylation process of pre-mRNA which is essential for producing mature mRNA molecules. PABPN1 operates within a multi-protein complex that includes enzymes like poly(A) polymerase and cleavage stimulation factor (CstF). By interacting with these proteins PABPN1 ensures the correct formation of the 3' end of mRNA influencing gene expression regulation and mRNA stability.
Pathways
PABPN1 participates in key cellular pathways such as mRNA processing and nuclear export of mRNA. It operates in concert with other proteins like PABPC1 which aids in the transition of mRNA from the nucleus to the cytoplasm. The protein maintains the balance of mRNA stability and translation efficiency which are critical aspects of efficient gene expression.
PABPN1 mutations are linked to oculopharyngeal muscular dystrophy (OPMD) a genetic condition that leads to muscle weakness. Another related disorder involves its interaction with proteins like HSP70 where its dysfunction may contribute to disrupted protein homeostasis in affected tissues. Studying PABPN1's role in these diseases provides insight into the molecular mechanisms underlying these conditions and aids in the development of potential therapeutic strategies.