Abcam, ab8189, Anti-PGP9.5 antibody [13C4 / I3C4] - Neuronal Marker

Size: 100µg
Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) is a mouse monoclonal antibody detecting PGP9.5 in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 130 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:13C4 / I3C4,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This antibody labels the neuronal cell bodies and axons in central and peripheral neural system. Small nerve fibers in peripheral tissues, neuroendocrine cells in normal pituitary thyroid, pancreas, and gastrointestinal tract, as well as derived tumors are also stained with this antibody.
Product Specifications
Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) is a mouse monoclonal antibody and is validated for use in ICC, IHC-P, WB in human, mouse, rat samples.
Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) specifically detects PGP9.5 (UniProt ID: P09936; Molecular weight: 25kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) has high sensitivity and specificity.
The specificity of Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) has been confirmed by testing in knockout samples.
Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) has been cited over 130 times in peer reviewed journals and is trusted by the scientific community.
Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) has 36 independent reviews from customers.
Related Products
Antibody clone 13C4 / I3C4 is also available pre-conjugated to a variety of labels for your convenience - HRP (
ab197735
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.
Biological function summary
PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.
Pathways
PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.
Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.