Abcam, ab82812, Anti-Calbindin antibody [CB-955]

Size: 100µL
Anti-Calbindin antibody [CB-955] (ab82812) is a mouse monoclonal antibody detecting Calbindin in Western Blot, IHC-P . Suitable for Cow, Human, Mouse, Rat . - Over 50 publications - Trusted since 2009
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CB-955,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat, Cow, Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Cow CALB1. The exact immunogen used to generate this antibody is proprietary information.P04467

Product details:
What is this antibody validated in?
Anti-Calbindin antibody [CB-955] (ab82812) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-Calbindin [CB-955] (ab82812) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-Calbindin [CB-955] (ab82812) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from hybridoma cell culture is Protein A column., Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calbindin also known as calbindin-D28k is a calcium-binding protein with a molecular mass of approximately 28 kDa. This target is mainly expressed in the neurons of the brain such as in the cerebellum and hippocampus as well as in the kidneys and intestine. Calbindin plays a mechanical role in binding and buffering intracellular calcium ions which is important for the regulation of calcium-dependent signaling pathways. Researchers often use techniques like Calbindin IHC (immunohistochemistry) and ELISA to study its distribution and presence within tissues.
Biological function summary
Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.
Pathways
Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.
Abnormal Calbindin expression has been observed in conditions like epilepsy and Parkinson's disease. In epilepsy its decreased expression can impact the ability of neurons to regulate calcium properly leading to increased vulnerability to seizures. In Parkinson's disease altered Calbindin levels have been associated with the selective vulnerability of dopamine neurons. These diseases highlight the connection between Calbindin and proteins like alpha-synuclein known for its role in Parkinson's pathology emphasizing the need to understand its contribution to disease mechanisms.