Abcam, ab8305, Anti-Insulin + Proinsulin antibody [D3E7]

Size: 250µg
Anti-Insulin + Proinsulin antibody [D3E7] (ab8305) is a mouse monoclonal antibody detecting Insulin + Proinsulin in IHC-Fr, ELISA . Suitable for Cow, Human, Mouse, Pig, Rat . - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:D3E7,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human, Pig,
Applications:ELISA, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Kd for this antibody is 6.3 x 10-8M.This antibody is specific for both insulin and proinsulin

Product details:
What is this antibody validated in?
Anti-Insulin + Proinsulin antibody [D3E7] (ab8305) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr), ELISA in Cow, Human, Mouse, Pig, Rat samples.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Insulin often referred to as 'insulin' is a peptide hormone with a mass of approximately 5.8 kDa generated by the beta cells in the pancreas. Its precursor proinsulin is synthesized as a larger molecule with a mass of about 9 kDa. Both insulin and proinsulin are primarily expressed in the pancreatic islets of Langerhans. Proinsulin undergoes enzymatic cleavage to form mature insulin and C-peptide. Insulin plays an essential role in glucose metabolism by regulating cellular uptake of glucose.
Biological function summary
Insulin and proinsulin are indispensable for maintaining glucose homeostasis in the body. Insulin binds to its receptor a receptor tyrosine kinase on target cells like muscle and adipose tissue to facilitate glucose uptake. Proinsulin also has a lesser-known role in insulin regulation and folding. It mostly exists independently rather than being part of a complex with insulin but precursor and mature insulin forms can transiently appear together during synthesis and secretion.
Pathways
Insulin function fits significantly into the signaling pathways such as the insulin signaling pathway and the PI3K/AKT pathway. These pathways importantly involve glucose transporters like GLUT4 and proteins such as IRS-1 that mediate insulin's effects on glucose and lipid metabolism. Insulin signals lead to the translocation of GLUT4 to the plasma membrane enhancing glucose uptake. Therefore insulin is closely related to these critical metabolic pathways.
Dysfunctions in insulin and proinsulin levels or actions are linked to conditions like diabetes mellitus and metabolic syndrome. In diabetes insulin resistance or deficiency leads to uncontrolled blood glucose levels highlighting the importance of insulin in glucose regulation. The link between insulin and obesity often involves dysregulation in insulin signaling pathways contributing to metabolic complications. Understanding these processes and their diseases is vital for developing therapeutic strategies.