Abcam, ab83353, Recombinant HIV1 tat protein

Size: 50µg / 100µg
Recombinant HIV1 tat protein is a Full Length protein, in the 1 to 101 aa range with >90% purity and suitable for western blot, SDS-PAGE, and Blocking assays. The predicted molecular weight of ab83353 protein is 20.1 kDa. - Recombinant HIV-1 Tat Clade C - Save time and ensure accurate results- use our HIV1 tat protein as a control - Available in different sizes to fit your experimental needs
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:BL, SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Animal free:No,
Carrier free:No,
Reconstitution:Reconstitute in PBS,
Storage buffer:Constituents: 0.1% Glycerol (glycerin, glycerine)

Product details:
Ensure the validity of your result using our recombinant HIV1 tat protein ab83353 as a positive control in western blot and SDS-PAGE. The HIV-1 TAT protein Clade C is a molecule of 101 amino acids encoded by two exons. ab83353 reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
This is a biologically non-active protein estimated by Up-Take and Rescue assays (positive to uptake assay but negative to rescue assay).
Check out our protein gel staining guide for SDS-PAGE
Check out of western blot protocol for more information

Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HIV-1 Tat protein also known as HIV Tat or simply Tat is an important regulatory protein of the Human Immunodeficiency Virus type 1 (HIV-1). It has a mass of approximately 14 to 16 kDa and is important for viral replication. This protein is expressed early in the HIV-1 infection cycle and is localized mainly in the nucleus of infected T-cells. HIV-1 Tat is known for its ability to transactivate the HIV-1 long terminal repeat (LTR) promoter which significantly enhances the production of viral RNA.
Biological function summary
The HIV-1 Tat protein plays an important role in the replication and transcription processes of the HIV-1 virus. It is part of a complex that interacts with other host cellular factors to improve the efficiency of the HIV-1 transcription from the provirus. This interaction is essential for the elongation phase of transcription which results in increased viral gene expression and successful replication of the virus inside host cells.
Pathways
The involvement of the HIV-1 Tat protein extends to interfering with several important biological pathways. It influences the NF-kB pathway which is critical for immune response regulation and interacts with the Cyclin T1 as part of the P-TEFb complex. This interaction is important for the transcriptional activation of the HIV LTR. By affecting the NF-kB pathway Tat protein indirectly modulates inflammatory responses which can lead to altered immune system functions.
HIV-1 Tat protein's primary significance lies in the progression and pathology of HIV/AIDS. Its interactions with host cellular proteins such as CDK9 (a component of P-TEFb) are critical for viral persistence and pathogenesis. Additionally Tat has been implicated in neurological disorders associated with HIV-1 infection often referred to as HIV-associated neurocognitive disorders (HAND). Its influence on the central nervous system arises from its ability to exit infected cells and exert neurotoxic effects in neighboring uninfected neural cells.