Abcam, ab83375, Sialic Acid (NANA) Assay Kit

Size: 100Test
Sialic Acid (NANA) Assay Kit ab83375 is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Cell culture media, Cell culture supernatant,
Assay type:Quantitative,
Sensitivity:= 1 µM,
Range:0.1 - 10 µM,
Assay time:40m,
Assay Platform:Microplate reader

Product details:
Sialic Acid (NANA) Assay Kit (ab83375) is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.
The sialic acid assay uses an enzyme coupled reaction in which oxidation of free sialic acid creates an intermediate that reacts stoichiometrically with the probe to generate a product that can detected by absorbance (OD = 570 nm) or fluorescence (Ex/Em=535/587 nm).
The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 uM concentration.
Other Notes
This product was previously called K566 Biovision Sialic Acid (NANA) Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C