Abcam, ab83501, Anti-Ku70 antibody

Size: 100µg
Rabbit Polyclonal Ku70 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 17 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ku70 also known as XRCC6 or K331 is a DNA repair protein with a molecular mass of approximately 70 kDa. It belongs to the Ku protein family which includes Ku80. This protein expresses mainly in the nucleus of cells across various tissue types. As a part of a heterodimer together with Ku80 Ku70 forms the Ku70/Ku80 complex which binds to free DNA ends allowing DNA repair processes to occur efficiently.
Biological function summary
The Ku70 protein plays a significant role in maintaining genomic stability. It is an important component of the non-homologous end joining (NHEJ) pathway a primary mechanism for repairing double-strand breaks in DNA. By forming the Ku70/Ku80 complex it accurately aligns DNA ends to facilitate repair and prevent chromosomal abnormalities. The Ku70 protein also involves in telomere maintenance and can influence cell cycle regulation.
Pathways
Ku70 contributes mainly to the NHEJ DNA repair pathway. This pathway is important for cellular response to DNA damage and involves other proteins such as DNA-PKcs (DNA-dependent protein kinase catalytic subunit) and XRCC4. Ku70's interaction with DNA-PKcs enables the activation of its kinase activity which is essential for the repair process. Additionally Ku70 is involved in the homologous recombination pathway where it interacts with proteins like Rad51 to ensure repair fidelity.
Research links the Ku70 protein to cancer and neurodegenerative diseases. Dysregulation or mutations affecting Ku70 can lead to genomic instability contributing to cancer development. Ku70 also shows connections to neurodegenerative conditions like Alzheimer's disease where its role in DNA repair and apoptosis regulation may impact neuronal cell survival. Interactions with p53 a protein important for DNA damage response further underline Ku70's influence in these disease contexts.