Abcam, ab83695, Human IL-27 ELISA Kit

Size: 1 x 96Tests / 2 x 96Tests
Human IL-27 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-27 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 12.8 pg/mL,
Range:31.25 - 1000 pg/mL,
Assay time:3h 45m,
Assay Platform:Microplate

Product details:
Abcam's IL-27 Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-27 in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific IL-27 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-27 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-27 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-27 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-27-A and IL-27-B.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C