Abcam, ab85077, Anti-AGO4 antibody

Size: 100µg
Rabbit Polyclonal AGO4 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AGO4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AGO4. The exact immunogen used to generate this antibody is proprietary information.Q9HCK5

Properties and Storage Information:
Form-Lyophilized, Reconstitution-reconstitute with water at 200µL, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.02% Sodium azideConstituents: 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Argonaute family member AGO4 also known as Argonaute RISC catalytic component 4 has a significant role in RNA-induced silencing complex (RISC) functions. AGO4's mass is approximately 95 kDa and it is primarily expressed in different tissues including the testes and placenta. AGO4 has been studied regarding its involvement in guiding microRNAs and short interfering RNAs in gene silencing through RNA interference.
Biological function summary
AGO4 participates in small RNA pathways. It is part of the RNA-induced silencing complex and works closely with DICER and TRBP to process and guide small RNA molecules. This process affects cellular mRNA levels by reducing the translation of target mRNA molecules helping regulate gene expression post-transcriptionally.
Pathways
AGO4 functions in the RNA interference and gene silencing by microRNAs pathways affecting post-transcriptional gene regulation. AGO4 interacts with proteins like DICER1 and other Argonaute proteins such as AGO1-3 within these pathways aiding in the effective downregulation of specific mRNA targets.
AGO4 has a connection to cancer and viral infections. Abnormalities in AGO4 expression or function may lead to disruption in gene silencing mechanisms which can contribute to tumor progression. Studies also link AGO4 to interactions with viral proteins highlighting its potential impact on viral replication and pathogenesis.