Abcam, ab85297, Recombinant human MEKK3 protein

Size: 10µg
Recombinant human MEKK3 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for WB, FuncS.
Key facts
Purity:>90% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity: 20nmol/min/mg.,
Accession:Q99759,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

Product details:
ab64311
(Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MEKK3 also known as MAP3K3 is a serine/threonine kinase that plays an important role in the MAPK signaling cascade. This protein has a molecular mass of approximately 67 kDa. MEKK3 is widely expressed with notable presence in tissues like the heart placenta and skeletal muscles. It is important for transmitting signals from the cell surface to various intracellular targets impacting numerous cellular processes.
Biological function summary
MEKK3 participates in cell growth differentiation and apoptosis regulation. It often functions within a multi-protein complex including components like MEK5 and ERK5. MEKK3 activates downstream kinases influencing gene expression and cellular responses to external signals. Its involvement is essential for controlling reactions to stress and inflammatory stimuli.
Pathways
MEKK3 integrates into the MAPK and NF-κB pathways both critical for mediating signaling responses. In the MAPK pathway MEKK3 regulates kinases like MEK5 and ERK5. Additionally it connects with proteins such as IKK in the NF-κB pathway influencing immunity and cell survival. These interactions position MEKK3 centrally in cellular defense and adaptability mechanisms.
MEKK3 associates with pathological conditions like cardiovascular diseases and cancer. Its role in these disorders links closely with dysregulation in the MAPK pathway where MEK5 and ERK5 also play significant roles. Furthermore aberrations in the NF-κB pathway involving IKK can contribute to its involvement in these diseases highlighting MEKK3's impact on disease progression and potential as a therapeutic target.