Abcam, ab85312, Anti-Robo1 antibody

Size: 100µg
Rabbit Polyclonal ROBO1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Roundabout homolog 1 (Robo1) also known as DUTT1 is a transmembrane receptor protein weighing approximately 181 kDa. It is part of the Robo receptor family specifically involved in axon guidance and neuronal migration. Robo1 primarily appears in neuronal tissues and other tissues like the lungs kidneys and prostate. Its role involves dynamic interactions with several ligands such as Slit proteins leading to the modulation of cellular pathways.
Biological function summary
Robo1 interacts with Slit proteins to control processes like repulsion of axonal growth cones during neuronal development. The protein acts as an important player in guiding neuronal connections and establishing boundaries. Robo1 does not function alone forming complexes with other receptors and cell adhesion molecules to execute its biological roles effectively. Structurally this receptor facilitates cellular responses to environmental cues through its cytoplasmic signaling cascade.
Pathways
Robo1 engages in the Slit-Robo signaling pathway essential for axonal guidance and cell migration. This pathway involves several key interactions with proteins such as DCC (Deleted in Colorectal Carcinoma) and Netrin impacting neuronal positioning and differentiation. Additionally Robo1 influences cellular orientation and tissue architecture by participating in the actin cytoskeleton remodeling pathway further demonstrating its significance in neuronal patterning.
Robo1 has links to cancer and neurodevelopmental disorders like autism spectrum disorders. The dysregulation of Robo1 expression has associations with aggressive tumor growth particularly in breast and lung cancers where altered signaling influences metastatic potential. Furthermore Robo1 interacts with proteins like Slit2 in cancer progression highlighting its importance in these conditions. In neurodevelopmental disorders altered Robo1 signaling can affect neural circuit formation contributing to changes seen in conditions like autism.