Abcam, ab85670, Anti-Spry-2 antibody

Size: 100µg
Rabbit Polyclonal Spry-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 11 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab180527.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Spry-2 also known as Sprouty homolog 2 is an intracellular protein involved in the regulation of signaling pathways. It has a molecular weight of approximately 35 kDa. This protein expresses in tissues such as the lung kidney and colon. Mainly Spry-2 acts through interaction with other proteins to inhibit or modulate various cellular processes such as growth and differentiation.
Biological function summary
Sprouty homolog 2 functions by mediating negative feedback in signaling pathways. It plays a role in controlling cellular responses to growth factors. Although not part of a large protein complex it interacts with specific proteins to exert its biological effects. For instance Spry-2 interacts with components of the receptor tyrosine kinase signaling pathway. This interaction helps fine-tune cell signaling responses preventing excessive cell proliferation.
Pathways
Sprouty homolog 2 acts within the MAPK/ERK signaling pathway and the fibroblast growth factor (FGF) pathway. In these pathways Spry-2 functions as an inhibitor thereby maintaining proper cellular regulation. It interacts with proteins like Raf and inhibits their activities ensuring balanced signal transduction. In the MAPK/ERK pathway this regulation by Spry-2 helps control cellular responses to external stimuli.
Abnormalities in Sprouty homolog 2 function relate to cancer and congenital anomalies. Altered expression or mutations in Spry-2 have associations with colorectal cancer partly due to its role in cell signaling regulation. Spry-2 connects to proteins like Ras known for their involvement in oncogenic pathways. Similarly variations in Spry-2 activity can lead to developmental disorders highlighting its importance in normal cellular function.