Abcam, ab8592, Anti-Desmin antibody

Size: 500µL
Rabbit Polyclonal Desmin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 109 publications. Immunogen corresponding to Full Length Protein corresponding to Human DES.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Human DES. The exact immunogen used to generate this antibody is proprietary information.P17661,
Specificity:In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.

Product details:
Prolonged fixation in buffered formalin can destroy the epitope.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.
Biological function summary
Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.
Pathways
Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.
Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.