Abcam, ab85950, Anti-GRLF1 antibody

Size: 100µL
Rabbit Polyclonal GRLF1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARHGAP35 aa 1200-1250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARHGAP35 aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information.Q9NRY4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab85950 was affinity purified using an epitope specific to GRLF1 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRLF1 also known as Glucocorticoid Receptor DNA Binding Factor 1 is a protein with a mass of approximately 196 kDa. This protein plays a role in gene regulation by interacting with DNA binding sites associated with glucocorticoid receptors. GRLF1 is widely expressed in various tissues but shows a higher expression level in the brain. It functions in stabilizing the linkage between glucocorticoid receptors and chromatin facilitating important transcriptional processes.
Biological function summary
GRLF1 interacts with nuclear receptors to regulate gene transcription and modulates cellular responses to glucocorticoids. It serves as a co-factor aiding in the assembly of transcriptional complexes needed for hormonal regulation. By forming complexes with other transcription factors GRLF1 influences cellular differentiation and immune response. Its involvement in the regulation of gene expression suggests a significant impact on cell signaling and homeostatic balance.
Pathways
GRLF1 contributes to the glucocorticoid signaling pathway and the MAPK/ERK pathway. In the glucocorticoid pathway it cooperates with the GR protein family to adapt cellular responses to stress and inflammation. Within the MAPK/ERK pathway GRLF1 assists in regulating cell growth and differentiation by interacting with proteins like ERK1/2 impacting cell cycle progression.
Researchers link GRLF1 to neurological disorders and inflammatory conditions. In neurodegenerative diseases altered GRLF1 function may affect neuronal survival and cognitive function. Its dysregulation can also contribute to autoimmune disorders where the inflammatory response becomes imbalanced. Through these connections GRLF1 interacts with proteins such as GR and NF-kB influencing both normal physiology and pathological states.