Abcam, ab85996, Anti-Kinetochore antibody

Size: 100µL
Rabbit Polyclonal Kinetochore antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KNTC1 aa 350-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human KNTC1 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.P50748

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab85996 was affinity purified using an epitope specific to Kinetochore immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The kinetochore is a protein complex that plays an important role during cell division particularly in ensuring proper chromosome segregation. It attaches to the centromere of each chromosome and serves as the anchor point for spindle microtubules. This interaction is essential for the movement and alignment of chromosomes on the metaphase plate. Although its exact molecular mass can vary due to its complex nature kinetochore is typically composed of multiple proteins including CENP proteins. It is found within the eukaryotic cells specifically expressed during mitosis and meiosis.
Biological function summary
Kinetochore is integral in forming a protein structure known as the kinetochore complex which is also called the spindle assembly checkpoint complex. It ensures chromosomes are properly aligned before the cell progresses to anaphase. Kinetochore also signals to delay anaphase until all chromosomes are correctly bi-oriented. Within this complex kinetochore proteins work together with regulatory proteins to prevent chromosome missegregation.
Pathways
Kinetochore plays an essential role in cell cycle regulation and mitotic spindle checkpoint pathways. It interacts closely with Aurora B and MAD2 proteins which both regulate the progression through mitosis. The mitotic spindle checkpoint pathway ensures chromosomes do not prematurely separate therefore maintaining genomic stability.
Kinetochore has a connection to conditions related to aneuploidy and cancer. Defective kinetochore function can lead to chromosome missegregation contributing to aneuploidy a feature observed in various cancers. Furthermore kinetochore malfunction is linked to Alzheimer's disease through its interaction with the tau protein which plays a role in neurodegenerative processes.