Abcam, ab86104, Anti-Cdc20 antibody

Size: 100µL
Rabbit Polyclonal CDC20 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Cell division cycle protein 20 homolog aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Cell division cycle protein 20 homolog aa 50-100. The exact immunogen used to generate this antibody is proprietary information.Q12834

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.
Biological function summary
Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.
Pathways
Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.
Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.