Abcam, ab86501, Anti-Islet 1 antibody [1B1] - Neural Stem Cell Marker

Size: 100µL
Anti-Islet 1 antibody [1B1] (ab86501) is a mouse monoclonal antibody detecting Islet 1 in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, . - KO validated for confirmed specificity - Trusted since 2009
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1B1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ISL1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P61371,
Specificity:In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands.

Product details:
What is this antibody validated in?
Anti-Islet 1 antibody [1B1] (ab86501) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, samples.
What is the molecular weight of Islet 1?
Anti-Islet 1 [1B1] (ab86501) specifically detects a band for Islet 1 (UniProt: P61371) at a molecular weight of 39kDa.
Specificity confirmed
The specificity of Anti-Islet 1 antibody [1B1] (ab86501) has been confirmed by Western blot testing in ISL1 Knockout HAP1 cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azide Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Islet 1 also known as ISL1 is a LIM-homeodomain transcription factor with a molecular mass of approximately 38 kDa. It plays an important role in regulating gene expression during the development of the nervous system and pancreas. You can find Islet 1 predominantly in motor neurons pancreatic beta cells and cardiac muscle cells. Its expression is important during embryonic stages for cell differentiation and maturation. Researchers use "Isl 1 neuronal marker" antibodies to identify this protein's presence in tissue.
Biological function summary
Islet 1 is involved in the differentiation and survival of various cell types. As part of a transcription complex it regulates downstream target genes essential for the development of motor neurons and cardiac muscle. It also contributes significantly to the developmental pathways of insulin-producing beta cells in the pancreas. The ISL1 marker is therefore an important tool for studying cell lineage and fate in developmental biology.
Pathways
Islet 1 is involved in key developmental signaling pathways including the Notch signaling and Wnt signaling pathways. In these pathways Islet 1 collaborates with other proteins like Lhx3 and Ngn2 to ensure proper gene activation for neuronal and cardiac development. Wnt signaling affects the expression of isl 1 marker genes contributing to precise spatial and temporal regulation during embryogenesis.
Islet 1 has links to congenital heart defects and type 2 diabetes. Mutations or altered expression of this protein can disrupt pancreatic beta cell function and motor neuron development. In congenital heart disease the interaction of Islet 1 with proteins like Nkx2.5 is critical. Its role in type 2 diabetes ties with the functioning of other proteins involved in insulin secretion making it a potential target for therapeutic intervention in these conditions.