Abcam, ab87913, Anti-Coilin antibody [IH10]

Size: 100µg
Anti-Coilin antibody [IH10] (ab87913) is a mouse monoclonal antibody detecting Coilin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P . Suitable for Human . - KO validated for confirmed specificity - Over 30 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:IH10,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:ICC, WB, Flow Cyt (Intra), IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Coilin antibody [IH10] (ab87913) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of Coilin?
Anti-Coilin [IH10] (ab87913) specifically detects a band for Coilin (UniProt: P38432) at a molecular weight of 63kDa.
Trusted by the scientific community
Anti-Coilin [IH10] (ab87913) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-Coilin antibody [IH10] (ab87913) has been confirmed by Western blot testing in COIL Knockout HeLa cell line,
ab261757
Other related products
We have a range of other formats of antibody clone [IH10] also available for your convenience: ab87913, Alexa Fluor® 647 -
ab196714
, HRP -
ab196715
, Alexa Fluor® 488 -
ab197531
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Coilin also known as Coilin protein or its gene name COIL is a nuclear phosphoprotein with a mass of approximately 62 kDa. It plays a mechanical role in the formation and maintenance of Cajal bodies which are subnuclear structures involved in various RNA processes. Coilin is mainly expressed in the nucleus of eukaryotic cells including a high expression in HeLa cells where its localization to Cajal bodies can be easily studied with tools like Alexa Fluor 488. Occasionally references may be made to terms like 'HeLa coil' which pertain to specific structural features noted in these contexts.
Biological function summary
This protein facilitates the organization and biogenesis of nuclear bodies associated with RNA processing and small nuclear ribonucleoproteins (snRNPs) assembly. Coilin operates as part of a larger complex involving several other proteins that interact within Cajal bodies. These nuclear bodies serve as sites where components necessary for RNA processing are concentrated. Coilin aids in the trafficking and modification of snRNP elements which are essential for pre-mRNA splicing.
Pathways
Coilin affects RNA metabolism and processing notably within the spliceosome assembly pathway and in snRNP biogenesis. The pathways include interactions with proteins such as SMN (Survival of Motor Neuron) protein which links to spinal muscular atrophy-related processes. Additionally Coilin contributes to the broader framework of nucleic acid processing systems impacting the outcome of RNA transcription and modification through its regulatory roles.
Coilin shows connections to neurological conditions and cancer. For instance alterations or dysregulation in Coilin expression can correlate with spinal muscular atrophy where it associates with the SMN protein if there are disruptions in Cajal body integrity. Furthermore links exist with certain cancers where the mismanagement of RNA processes influenced by Coilin and its interaction partners like PDEdelta can lead to aberrant cell behaviors and tumor progression. Coilin’s involvement in such diseases makes it an appealing target for therapeutic exploration reflected in ongoing cancer research and therapeutic developments.