Abcam, ab88878, Anti-EME1 antibody

Size: 50µg
Mouse Polyclonal EME1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EME1.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human EME1.Q96AY2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The EME1 protein also known as Essential Meiotic Endonuclease 1 plays a mechanical role in the DNA repair process. It has a molecular mass of approximately 58 kDa. EME1 is ubiquitously expressed in various tissues which suggests its broad importance in cellular functions. Mechanically it forms part of the XPF-EME1 endonuclease complex where it partners with the XPF protein to facilitate repair of DNA interstrand crosslinks and the resolution of DNA joint molecules during homologous recombination.
Biological function summary
EME1 participates in maintaining genomic stability. As a component of the XPF-EME1 complex it executes incision of branched DNA structures important for DNA repair processes such as nucleotide excision repair and interstrand crosslink repair. Additionally it contributes to safeguarding the integrity of replicating DNA. Through these biological actions EME1 ensures the prevention of genome instability which could otherwise lead to cellular dysfunction.
Pathways
EME1 operates within the DNA repair pathways notably the nucleotide excision repair (NER) and the homologous recombination repair (HRR) pathways. In NER EME1 partners with the XPF protein enabling precise corrections of damaged DNA. In the HRR pathway EME1 interacts with other repair proteins to facilitate appropriate chromosomal segregation and DNA replication. Consequently its action underpins these pathways' essential roles in fixing DNA damages and maintaining genetic accuracy.
Mutations or dysfunctions in EME1 have links to increased susceptibility to cancers and genomic instability disorders like Fanconi anemia. The protein's integral role in DNA repair connects it to several other proteins such as BRCA2 which collaborates with EME1 in homologous recombination. When EME1 function is impaired these collaborations break down contributing to the pathogenesis of these conditions.