Abcam, ab89396, Anti-CCL21 antibody [MM0146-2H26]

Size: 100µg
Mouse Monoclonal CCL21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL21.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MM0146-2H26,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human CCL21.O00585

Product details:
Reconstituted antibody should be aliquoted and can be stored frozen at < -20 for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Form-Lyophilized, Reconstitution-reconstitute with PBS at 200µL, Purification technique-Affinity purification Protein G, Purification notes-The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 μm filter., Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCL21 also known as Exodus 2 or 2H26 is a chemokine with significant roles in the immune system. It is a small protein with a molecular mass of approximately 12 kDa. CCL21 is expressed in high concentrations in secondary lymphoid organs such as the spleen lymph nodes and Peyer's patches. It serves as a chemoattractant particularly for lymphocytes and dendritic cells facilitating their migration towards lymphoid organs.
Biological function summary
CCL21 is essential for guiding immune cells to areas where they are needed for initiating immune responses. It forms a non-covalent complex with its receptor CCR7 a primary mechanism through which it exerts its biological effects. CCL21 coordinates the positioning of immune cells within the lymphoid organs ensuring a functional and effective immune response to pathogens or injuries. Its expression pattern aids in the organization of lymphoid tissue architecture and contributes to efficient trafficking of T-cells and dendritic cells.
Pathways
CCL21 plays an important role in the chemokine signaling pathway and the leukocyte transendothelial migration pathway. Within these pathways CCL21 works closely with its receptor CCR7 to guide immune cell navigation and localization. This collaboration is essential for directing cells to appropriate sites in the immune system aligning CCL21 with similar proteins like CCL19 another chemokine that also interacts with CCR7.
CCL21 is associated with several immune-related conditions including rheumatoid arthritis and chronic lymphocytic leukemia. In rheumatoid arthritis CCL21 contributes to the infiltration and retention of immune cells in the synovial tissue while in chronic lymphocytic leukemia it aids in the homing of malignant cells to lymphoid organs. CCL21's interaction with CCR7 in these diseases highlights its importance not only in immune surveillance but also in the pathology of these disorders.