Abcam, ab8980, Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker

Size: 100µg
Anti-Lamin A antibody [133A2] (ab8980) is a mouse monoclonal antibody detecting Lamin A in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Over 90 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:133A2,
Isotype:IgG3,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, Flow Cyt, IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human LMNA. The exact immunogen used to generate this antibody is proprietary information.P02545,
Epitope:Recognizes an epitope located between residues 598-611 of lamin A.,
Specificity:Reacts against lamin A exclusively (the antibody was raised against the carboxy-terminus of 98 amino acids present in lamin A and absent from lamin C. As a result, this antibody recognizes lamin A but not lamin C; See Machiels et al 1997). Lamins do not appear to be universally distributed among different cell and tissue types. ab8980 has been tested in testis parenchyma and testicular germ cell tumours (See Machiels et al 1997). Other cell/tissue types have not been tested.

Product details:
What is this antibody validated in?
Anti-Lamin A antibody [133A2] (ab8980) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of Lamin A?
Anti-Lamin A [133A2] (ab8980) specifically detects a band for Lamin A (UniProt: P02545) at a molecular weight of 74kDa.
Trusted by the scientific community
Anti-Lamin A [133A2] (ab8980) was first used in a scientific publication in 2002 and has been cited over 90 times in peer-reviewed journals.
Reviewed by scientists
Anti-Lamin A [133A2] (ab8980) has over 15 independent reviews from customers.
Specificity confirmed
The specificity of Anti-Lamin A antibody [133A2] (ab8980) has been confirmed by Western blot testing in LMNA Knockout HAP1 cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin A also known as LMNA is a type of lamin protein integral to the nuclear architecture. It has a molecular weight of approximately 70 kDa. This protein localizes mainly to the nuclear lamina a dense fibrillar network inside the inner nuclear membrane found across various cell types. Lamin A serves as a structural scaffold mediating nuclear stability and rigidity. It also plays a role in chromatin organization supporting overall nuclear functions.
Biological function summary
The lamin molecule interacts with several nuclear components forming part of the nuclear lamina complex. It anchors chromatin material and regulates DNA replication and repair. Lamin A protein also modulates gene expression through interactions with transcription factors. Beyond structural support it influences cell cycle progression and differentiation impacting cellular mechanotransduction and signaling processes.
Pathways
Lamin A performs critical functions within the cell cycle and apoptotic pathways. Its interactions with the retinoblastoma protein (pRB) and other cyclin-dependent kinases control cell cycle checkpoints and progression. Moreover lamin A connects with proteins involved in signaling pathways like MAPK which relate to stress responses and cellular growth. These interactions highlight its dynamic involvement in maintaining cell health and proliferation.
Mutations in the lamin A gene associate closely with Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. In these conditions altered lamin A protein affects nuclear shape and chromatin layout disrupting transcriptional regulation. The mutated lamin A protein interacts differently with binding partners like emerin contributing to muscle adipose tissue and multi-system abnormalities. This highlights the critical role lamin A plays in maintaining normal cellular function across life stages.