Abcam, ab89901, Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

Size: 20µL / 100µL / 1mL
Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) is a rabbit monoclonal antibody detecting Wilms Tumor Protein in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 250 publications - Trusted since 2010
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:CAN-R9(IHC)-56-2,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-P, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Expression levels of the target protein vary with sample type and some optimisation may be required.

Product details:
Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) was first used in a scientific publication in 2011 and has been cited over 252 times in peer reviewed journals. It's performance in IHC in mouse and human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) has 40 independent reviews from customers.
Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) specifically detects Wilms Tumor Protein (UniProt ID: P19544; Molecular weight: 50kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone CAN-R9(IHC)-56-2 -
ab216646
Antibody clone CAN-R9(IHC)-56-2 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, FITC, Alexa Fluor
ab202635
ab202639
ab223934
ab314587
Wilms Tumor Protein (WT1) is a tumor-associated antigen that is overexpressed in various cancers, making it a target for immunotherapeutic approaches. It plays a role in tumor progression and immune evasion, influencing the tumor microenvironment and the effectiveness of immune responses.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Wilms Tumor Protein commonly known as WT1 is a critical transcription factor implicated in several cellular processes. It is also identified by other names such as Wilms Tumor Suppressor and WT33. WT1 protein has a mass of approximately 52-54 kDa. It is broadly expressed in various tissues with high levels in the developing kidney gonads and certain mesothelial tissues. Its expression plays an important role in organ development and cellular differentiation.
Biological function summary
The WT1 protein regulates gene expression by binding to specific DNA sequences influencing cell growth and differentiation. WT1 acts as a part of larger protein complexes interacting with other transcription factors and co-regulators. It plays essential roles in the development of the urogenital system and in the maintenance of mesothelial cells. The WT1 protein is essential for normal kidney and gonadal development highlighting its significance in embryogenesis.
Pathways
WT1 integration involves multiple cellular signaling cascades. It is notably engaged in the Wnt and PI3K/AKT signaling pathways which are critical for cell proliferation and survival. In these pathways WT1 interaction with proteins such as β-catenin and PI3K subunits modulates cell fate decisions. This involvement in signaling networks exemplifies its importance in cellular homeostasis and response to environmental cues.
WT1 mutations or dysregulation associate strongly with specific pathologies particularly Wilms tumor and acute myeloid leukemia (AML). In Wilms tumor WT1 acts as a tumor suppressor gene and its loss of function leads to tumorigenesis in the kidney. In AML aberrant WT1 expression affects normal hematopoiesis and is often linked to poor prognosis. Interaction with proteins like BSA in immunohistochemistry diagnostics highlights WT1’s significance as a biomarker for these diseases.