Abcam, ab90649, Anti-DGCR8 antibody

Size: 100µg
Rabbit Polyclonal DGCR8 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DGCR8 aa 400-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DGCR8 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.Q8WYQ5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DGCR8 protein also known as Pasha in some organisms serves an important role in the microRNA (miRNA) processing machinery. It has a molecular mass of approximately 90 kDa and shows expression ubiquitously but with higher levels in tissues with active cell division like the brain and lungs. DGCR8 acts as a partner in a complex with Drosha and together they initiate the microRNA maturation process by cleaving primary miRNA transcripts into precursor miRNA in the nucleus.
Biological function summary
DGCR8 partners with Drosha to form the Microprocessor complex an important player in miRNA biogenesis. This complex identifies and accurately processes primary transcripts into precursor miRNAs a step essential for proper gene regulation. DGCR8's role is critical for maintaining the normal cellular function enabling the precise control of miRNA production which in turn modulates gene expression post-transcriptionally. Aberrations in its function affect the regulation of genes involved in cell cycle differentiation and development.
Pathways
DGCR8 holds a significant influence on the RNA interference (RNAi) pathway interacting closely with Drosha to regulate miRNA synthesis. This pathway is fundamental for the regulation of genetic networks involved in cellular development and homeostasis. In the context of the miRNA processing pathway DGCR8 links to proteins like Dicer which further processes precursor miRNAs in the cytoplasm. Proper function of these pathways ensures the balanced expression of mRNAn important for healthy cellular activities.
DGCR8 misregulation is associated with the development of DiGeorge syndrome and certain cancers. Alterations in its function can lead to compromised miRNA maturation impacting genes that control cell growth and survival. In DiGeorge syndrome haploinsufficiency of DGCR8 may contribute to the associated developmental anomalies. In cancer altered DGCR8 expression can disrupt miRNA profiles interacting further with proteins such as Argonaute to influence the dysregulation of gene expression profiles commonly observed in tumorigenesis.