Abcam, ab91447, Anti-NAS6 antibody

Size: 100µL
Rabbit Polyclonal Gankyrin antibody. Suitable for IP, WB and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C NAS6.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Saccharomyces cerevisiae,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C NAS6.P50086

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6 - 8.5Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein NAS6 also known as p28 and gankyrin is a regulatory component with an approximate mass of 28 kDa. NAS6 has significant expression in the human liver as well as detectable levels in several other tissues such as the brain and heart. Mechanically NAS6 interacts with the proteasome aiding in the regulation of protein degradation. The interaction with the proteasome involves NAS6 binding to specific subunits and helping in substrate recognition.
Biological function summary
NAS6 plays an important role in cellular protein homeostasis by associating with the 26S proteasome complex. This association facilitates the targeting and degradation of ubiquitinated proteins which is critical for maintaining cellular function. Through its activity NAS6 contributes to the removal of damaged or misfolded proteins and regulation of the cell cycle. This regulation includes controlling the abundance of cyclins and other proteins that are pivotal in cell division processes.
Pathways
NAS6 integrates into the ubiquitin-proteasome pathway a highly conserved pathway responsible for protein turnover in the cell. The pathway involves proteins like ubiquitin which tags proteins for degradation and the 26S proteasome where NAS6 plays its regulatory role. NAS6 also interacts with other proteins such as MDM2 in the p53 pathway linking its function to cellular stress responses and apoptosis regulation.
NAS6 has implications in oncogenesis particularly in liver cancer and colorectal cancer. Overexpression of NAS6 links to uncontrolled cell proliferation mostly due to its effect on protein degradation pathways. Additionally NAS6 interacts with the retinoblastoma protein (Rb) influencing pathways associated with tumorigenesis. Alterations in NAS6 levels or function can disrupt normal cellular regulation highlighting its relevance in cancer research and therapy development.