Abcam, ab91656, Anti-Apo-M antibody [EPR2904]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Apo-M antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2904,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein M (Apo-M) also known as protein G3a is a 25 kDa member of the lipocalin family which is secreted primarily by liver and kidney cells. Apo-M is a component of high-density lipoprotein (HDL) where it plays a significant role in lipid metabolism. It serves as a binding partner for lipophilic molecules such as sphingosine-1-phosphate (S1P) impacting the stability and functionality of HDL particles. Researchers use anti-Apo antibodies to investigate the expression localization and function of Apo-M in various conditions.
Biological function summary
Apo-M influences several critical processes. It binds with S1P which is involved in endothelial barrier function vascular development and immune cell trafficking. Apo-M is also important for maintaining the inverse relationship between cholesterol levels and cardiovascular risk. It functions within HDL complexes affecting cholesterol efflux and anti-inflammatory responses. Apart from HDL Apo-M interacts with other Apo proteins like apoA-I and apoA-II serving as a regulatory component within these lipoprotein complexes.
Pathways
Apo-M integrates into lipid metabolism and signal transduction pathways. It participates in the S1P signaling pathway which is important for maintaining vascular integrity and immune function. Apo-M's interactions within this pathway influence the activity of other proteins such as the S1P receptors which play a critical role in mediating its biological activities. Additionally Apo-M interfaces with cholesterol efflux pathways interacting with the ATP-binding cassette transporters particularly ABCA1 that facilitate cholesterol removal from peripheral tissues.
Apo-M has associations with atherosclerosis and metabolic syndrome. Dysregulation of Apo-M levels can contribute to the development of atherosclerotic plaques as it affects HDL's protective roles against oxidation and inflammation. Alterations in Apo-M levels may also influence metabolic syndrome components given its function in lipid metabolism and glucose homeostasis. In these contexts changes in Apo-M can affect proteins like apoE which are involved in lipid transport and cardiovascular risk modulation.