Abcam, ab9225, Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

Size: 100µg
Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) is a mouse monoclonal antibody detecting M13 Bacteriophage Coat Protein g8p in Western Blot, Flow Cytometry . Suitable for Enterobacteria phage M13 . - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:RL-ph2,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Enterobacteria phage M13,
Applications:ICC, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Enterobacteria phage M13 Capsid protein G8P.P69541,
Specificity:RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.

Product details:
What is this antibody validated in?
Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt) in Enterobacteria phage M13 samples.
What is the molecular weight of M13 Bacteriophage Coat Protein g8p?
Anti-M13 Bacteriophage Coat Protein g8p [RL-ph2] (ab9225) specifically detects a band for M13 Bacteriophage Coat Protein g8p (UniProt: P69541) at a molecular weight of 5kDa.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The M13 bacteriophage coat protein g8p also known simply as g8p plays a mechanical role in forming the protective sheath around the phage's single-stranded DNA. The molecular weight of g8p is approximately 5 kDa. It is an important component of the M13 bacteriophage typically expressed within the bacterial host after phage infection. As one of the coat proteins g8p drives the assembly of new phage particles by arranging itself into helical structures that encapsulate the viral genome.
Biological function summary
G8p's function extends beyond just structural support for the phage. It participates in the assembly of the M13 phage which is part of the extrinsic complex necessary for phage propagation. During this process g8p cooperates closely with other proteins to manage the packaging of genetic material and ensure successful phage extrusion from the bacterial host. Functionally g8p aids in maintaining phage integrity and viability directly affecting the efficiency of phage propagation within the microbial environment.
Pathways
G8p has an essential role in the M13 bacteriophage lifecycle. It facilitates the phage's extrusion pathway where newly formed phage particles exit the bacterial cell. This pathway involves interactions with proteins like the M13 p9 (g9p) protein ensuring efficient phage release and infection of nearby bacterial cells. The interaction among these proteins through the extrusion pathway is critical for the replication and spread of the M13 bacteriophage.
G8p as a component of phage display technology interacts indirectly with a variety of disorders particularly bacterial infections. M13 phage display systems use g8p to present peptides or proteins on the phage surface aiding in identifying molecules that can target infectious agents. Although g8p's primary function does not link directly to specific diseases its application in phage display connects it with therapeutic and diagnostic developments potentially interacting with proteins involved in bacterial pathogenesis.