Abcam, ab92334, Anti-WIZ antibody

Size: 100µg
Goat Polyclonal WIZ antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WIZ aa 550-650.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human WIZ aa 550-650. The exact immunogen used to generate this antibody is proprietary information.O95785,
Specificity:ab92334 detects isoform 2 WIZ

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WIZ also known as zinc finger protein 803 is a large protein with a mass of approximately 234 kDa. It plays a mechanical role as a transcriptional regulator particularly by binding to methylated DNA and interacting with proteins that modify chromatin. WIZ expresses in various tissues with higher levels in the brain and lymphoid tissues. It contains multiple zinc finger domains which facilitate its binding to DNA.
Biological function summary
The structure allows WIZ to influence gene expression by forming part of larger protein complexes. These complexes include known components such as the G9a/GLP histone methyltransferase. WIZ modulates chromatin structure by recruiting these complexes to specific genomic regions affecting methylation patterns and thereby regulating gene activity. This chromatin remodeling capability suggests its involvement in controlling cellular differentiation and proliferation.
Pathways
The function of WIZ is essential in pathways governing gene regulation and chromatin state. It integrates into the epigenetic pathway involving histone modification alongside related proteins like EHMT1 and EHMT2 also known as G9a and GLP. It also participates in neuronal developmental pathways influencing genes critical for brain development and function. These roles link WIZ to broader signaling networks that impact cellular growth and differentiation.
The activity of WIZ connects with conditions such as intellectual disability and certain cancers. Abnormalities in its function or expression can lead to altered gene expression profiles which associate with tumorigenesis and neurodevelopmental disorders. The target's connection to disorders includes interactions with proteins like EHMT1 which have reported mutations in cases of mental retardation indicating a shared pathway impact on disease mechanisms.