Abcam, ab9319, Anti-Phosphotyrosine antibody

Size: 100µg
Rabbit Polyclonal Phosphotyrosine antibody. Suitable for IP, ELISA, WB, IHC-P, IHC-Fr and reacts with Modified Amino Acid samples. Cited in 11 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:WB, IP, ELISA, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Recognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine., Storage buffer-pH: 6 - 8Preservative: 0.01% Sodium azideConstituents: 0.114% Tris buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.
Biological function summary
Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.
Pathways
The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.
The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.