Abcam, ab9439, Anti-L-Aspartate antibody

Size: 500µL
Rabbit Polyclonal L-Aspartate antibody. Suitable for ICC, IHC-FoFr, IM, IHC-Fr and reacts with Modified Amino Acid samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:ICC, IHC-Fr, IHC-FoFr, IMSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000)was detected against aspartate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Significant cross-reactivity (1:10) was detected against D-aspartate.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
L-Aspartate known as aspartate or L-arginine L-aspartate is an amino acid with a molecular mass of about 133.10 g/mol. This amino acid is expressed widely in various tissues and plays an important role in protein synthesis as well as other significant cellular functions. As a non-essential amino acid L-aspartate can be synthesized by the body and can also be obtained from dietary proteins. It participates in nitrogen metabolism and its presence is essential for the functioning of many metabolic cycles.
Biological function summary
L-aspartate contributes to energy production and biosynthesis of nucleotides. It is often found participating in enzyme complexes and is involved in the citric acid cycle and urea cycle which are significant in energy metabolism and waste removal. L-aspartate also serves as a neurotransmitter influencing brain and nerve function. By regulating the synthesis of other amino acids such as asparagine it maintains cellular homeostasis.
Pathways
L-aspartate integrates into important metabolic pathways like the Krebs cycle and the urea cycle. It plays a role in gluconeogenesis and processes related to energy production. The Krebs cycle where L-aspartate aids in the conversion of carbohydrates fats and proteins into carbon dioxide and water for energy production involves related proteins such as oxaloacetate and malate. Through these pathways L-aspartate ensures the balance of energy and metabolites within cells.
L-aspartate connects to conditions like hepatic encephalopathy and hyperammonemia both characterized by imbalances in ammonia metabolism. In hepatic encephalopathy the liver's inability to convert ammonia into urea leads to toxicity affecting the brain. Related enzymes involved in ammonia processing such as carbamoyl phosphate synthetase correlate with L-aspartate's function in addressing these disorders. Understanding L-aspartate's roles and interactions helps illuminate potential therapeutic targets for these and other conditions.