Abcam, ab95310, Recombinant human PAK2 protein

Size: 10µg
Recombinant human PAK2 protein is a Human Fragment protein, in the 227 to 524 aa range, expressed in Escherichia coli, with >90%, suitable for FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:86 nmoles/min/μg Analysis of enzymatic activity: 1. Different concentrations of PAK2 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm filters.,
Accession:Q13177,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.395% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle