Abcam, ab9535, Anti-Myeloperoxidase antibody

Size: 500µL
Anti-Myeloperoxidase antibody (ab9535) is a rabbit polyclonal antibody detecting Myeloperoxidase in IHC-P . Suitable for Human . - Over 320 publications - Trusted since 2002
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Light chain type:unknown,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human MPO.P05164

Product details:
Anti-Myeloperoxidase antibody (ab9535) is a rabbit polyclonal antibody and is validated for use in ICC, IHC-P in human, mouse samples.
Anti-Myeloperoxidase antibody (Anti-MPO antibody) (ab9535) has been cited over 320 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Myeloperoxidase antibody (ab9535) has high sensitivity and specificity.
Anti-Myeloperoxidase antibody (ab9535) has 36 independent reviews from customers.
Anti-Myeloperoxidase antibody (ab9535) specifically detects Myeloperoxidase (UniProt ID: P05164; Molecular weight: 79kDa) and is sold in 500 µL selling sizes.
Myeloperoxidase (MPO) is an enzyme predominantly found in neutrophils, where it plays a critical role in the immune response by producing hypochlorous acid to kill pathogens. However, MPO can also contribute to tissue damage and inflammation, and its deficiency is linked to increased susceptibility to infections, particularly fungal infections like those caused by Candida species. MPOcontributes to inflammation by producing reactive oxygen species (ROS) and hypochlorous acid (HOCl) during the immune response. This enzyme's activity is linked to various inflammatory diseases, including atherosclerosis and neuroinflammation.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.3 - 7.5Preservative: 0.05% Sodium azideConstituents: 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.