Abcam, ab95397, Anti-NAPE-PLD antibody

Size: 500µL
Rabbit Polyclonal NAPE-PLD antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human NAPEPLD aa 150-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NAPEPLD aa 150-200. The exact immunogen used to generate this antibody is proprietary information.Q6IQ20

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NAPE-PLD also known as N-acyl phosphatidylethanolamine phospholipase D is an enzyme with a molecular mass of approximately 46 kDa. It functions in hydrolyzing N-acyl phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) which include bioactive lipids like anandamide. NAPE-PLD is expressed widely in mammalian tissues such as the brain and liver indicating its broad physiological significance.
Biological function summary
NAPE-PLD plays an important role in generating bioactive lipids that act as signaling molecules. This enzyme is not known to be part of a larger protein complex but functions independently to influence lipid-based signaling pathways. By modulating the levels of NAEs it affects various physiological processes including inflammation and neuroprotection.
Pathways
The ability of NAPE-PLD to produce NAEs integrates it into the endocannabinoid signaling pathway a critical route for maintaining homeostasis in the body. It works alongside other enzymes like fatty acid amide hydrolase (FAAH) that break down NAEs. The activity of NAPE-PLD influences cannabinoid receptor activity impacting several pharmacological responses and regulatory mechanisms in the nervous system.
Involvement of NAPE-PLD is significant in conditions such as neurodegenerative diseases and metabolic disorders. Alterations in its activity have been linked to Alzheimer's disease where it affects endocannabinoid levels. The enzyme also connects to obesity-related disorders through its impact on energy balance and lipid metabolism alongside proteins like diacylglycerol lipase which also participate in lipid signaling networks.