Abcam, ab96328, Anti-POLR3A antibody

Size: 100µL
Anti-POLR3A antibody (ab96328) is a rabbit polyclonal antibody detecting POLR3A in Western Blot, IP, ChIP . Suitable for Human, Mouse . - Over 10 publications - Trusted since 2010
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human POLR3A aa 150-500. The exact immunogen used to generate this antibody is proprietary information.O14802

Product details:
What is this antibody validated in?
Anti-POLR3A antibody (ab96328) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ChIP in Human, Mouse samples.
What is the molecular weight of POLR3A?
Anti-POLR3A (ab96328) specifically detects a band for POLR3A (UniProt: O14802) at a molecular weight of 156kDa.
Trusted by the scientific community
Anti-POLR3A (ab96328) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
POLR3A also known as RNA polymerase III subunit A or RPC1 plays an important role in the transcription machinery of eukaryotic cells. It functions as the largest subunit of RNA polymerase III which is responsible for transcribing small non-coding RNAs such as tRNA and 5S rRNA. POLR3A has a molecular mass of approximately 155 kDa and its expression can be detected across various tissues reflecting its essential role in basic cellular functions.
Biological function summary
The primary function of POLR3A involves its role as part of the RNA polymerase III complex. This complex synthesizes tRNA and other small RNAs which are important for protein synthesis and various regulatory processes within the cell. POLR3A must work seamlessly with other subunits in the polymerase III complex to ensure efficient RNA production maintaining proper cellular function and growth.
Pathways
Transcription by POLR3A is vital for synthesis of precursor tRNAs in the transcription pathway. It also plays a part in the immune response pathway known as the cytosolic DNA sensing pathway where POLR3A has been linked to recognition of viral DNA. This involvement indicates a connection with proteins such as RIG-I and MAVS which help mediate responses to pathogenic infections.
Mutations in the POLR3A gene can lead to POLR3-related leukodystrophy a neurological disorder affecting white matter in the brain. Additionally variations in POLR3A have been implicated in certain types of systemic sclerosis an autoimmune disorder. These associations highlight the interplay of POLR3A with disease-related proteins such as POLR3B another subunit in the RNA polymerase III complex which can similarly impact cellular and systemic functions when mutated.