Abcam, ab9641, Anti-ORC4L antibody

Size: 100µg
Goat Polyclonal ORC4L antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human ORC4 aa 400 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ORC4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O43929

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The origin recognition complex subunit 4-like (ORC4L) also known simply as ORC4 is a mechanically important player in the initiation of DNA replication. ORC4 typically has a molecular mass of approximately 50 kDa. It is highly expressed in cells that are actively dividing especially within the nucleus where replication origins are located. The protein forms part of the larger origin recognition complex (ORC) which is important for marking the starting points for DNA replication across eukaryotic organisms.
Biological function summary
ORC4 plays a central role in recognizing replication origins and acts as an anchor for the assembly of other necessary replication proteins. It is one of six subunits that comprise the ORC complex. This protein complex binds to origins of replication in DNA unwinds the double helix and recruits additional factors required for the formation of the pre-replicative complex. The presence of ORC4 is essential in ensuring that DNA replication occurs once and only once per cell cycle preventing genomic instability.
Pathways
Several cellular processes depend on the correct functioning of ORC4. In cell cycle regulation ORC4 is integral to the DNA replication checkpoint which ensures DNA is not damaged before it is duplicated. ORC4 interacts with other replication factors like CDC6 and MCM2-7 helicase to initiate and regulate the replication process. Its activity influences key pathways such as the p53-dependent DNA damage response where ORC4 can affect decisions related to cell cycle arrest or apoptosis.
ORC4 dysregulation links to genomic instability which is a hallmark in the development of cancer. Altered levels or mutations in ORC4 contribute to an increased susceptibility to oncogenesis with links to various cancers such as those of the breast and colon. The BRCA1 protein known for its role in maintaining genomic stability and DNA repair often shows interactions with ORC4 during such disease states highlighting their interconnected roles in cancer-related pathways.