Abcam, ab9655, Anti-Bmf antibody

Size: 100µg
Rabbit Polyclonal BMF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human Bcl-2-modifying factor aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Bcl-2-modifying factor aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q96LC9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bmf also known as Bcl-2 modifying factor is important in apoptosis regulation a process of programmed cell death. It acts as a pro-apoptotic protein from the Bcl-2 family. The Bmf protein has a molecular weight of approximately 21 kDa. It is mainly expressed in various epithelial tissues and abundant in hematopoietic cells. Bmf can interact with the dynein light chain at the cytoskeleton which affects its localization and function.
Biological function summary
The protein exerts its function by promoting apoptosis. Bmf binds directly to pro-survival Bcl-2 family members such as Bcl-X_L and Bcl-2 displacing pro-apoptotic proteins like Bax or Bak. This action facilitates the release of cytochrome c from mitochondria triggering caspase activation and cell death. Bmf can function independently but also works within larger complexes affecting processes related to cellular homeostasis.
Pathways
Bmf operates prominently within the intrinsic apoptotic pathway where it modulates mitochondrial outer membrane permeability. Its action is closely associated with Bax and Bak proteins facilitating their oligomerization. Additionally Bmf integrates into stress-induced signaling pathways responding to signals like oxidative stress or deprivation of survival factors. Through these connections Bmf influences cell fate and stress responses often cross-talking with the JNK signaling pathway.
Bmf has implications in several types of cancer and neurodegenerative conditions. Overexpression or dysregulation of Bmf can contribute to tumor development due to impaired apoptosis. Specific cancers like lymphomas show altered Bmf levels linking it to pathways with proteins such as Bcl-2 and Bax. In neurodegenerative diseases changes in Bmf expression can impact neuronal survival with implications for conditions like Alzheimer's where its interaction with other Bcl-2 family proteins affects cell viability.