Abcam, ab96612, Anti-TRIM32 antibody

Size: 50µL
Rabbit Polyclonal TRIM32 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Human, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM32 aa 1-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TRIM32 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.Q13049

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRIM32 also known as Tripartite Motif Containing 32 is a protein that functions as an E3 ubiquitin ligase. It plays a role in attaching ubiquitin molecules to proteins tagging them for degradation by the proteasome. TRIM32 consists of a RING domain a B-box and a coiled-coil region which are characteristic of the TRIM family. The molecular mass of TRIM32 is approximately 71 kDa. It is expressed in various tissues with notable expression in skeletal muscle and the heart implying an important role in muscle physiology.
Biological function summary
TRIM32 participates in regulating muscle cell differentiation and proliferation. It interacts with several proteins including dysbindin and myospryn suggesting its involvement in a multi-protein complex. TRIM32 has a regulatory role in muscle maintenance where it influences the stability and function of muscle proteins. Moreover it is also involved in neurogenesis affecting neuronal differentiation and survival emphasizing its role beyond muscle tissues.
Pathways
TRIM32 plays a significant role in the PI3K/Akt signaling pathway and the TGF-beta signaling pathway. In the PI3K/Akt pathway TRIM32 modulates cell growth and survival interacting with proteins such as Akt and mTOR. In the context of TGF-beta signaling TRIM32 can influence cellular processes like proliferation and differentiation possibly by interacting with SMAD proteins. These interactions indicate its importance in cellular growth and differentiation pathways.
TRIM32 is linked with limb-girdle muscular dystrophy type 2H and certain forms of sarcopenia. Mutations in TRIM32 cause dysfunction in muscle protein turnover leading to muscle deterioration and weakness. In limb-girdle muscular dystrophy TRIM32 mutations disrupt its interaction with dysbindin impairing muscle structure. Furthermore TRIM32 might be involved in neurodegenerative diseases with connections to proteins like alpha-synuclein hinting at a potential role in Parkinson's disease.