Abcam, ab97351, Anti-XRCC4 antibody

Size: 50µL
Rabbit Polyclonal XRCC4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DNA repair protein XRCC4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.Q13426

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
XRCC4 also known as X-ray repair cross-complementing protein 4 is an important component in the repair of double-strand breaks in DNA. It stabilizes and facilitates the joining of DNA ends during the non-homologous end joining (NHEJ) repair pathway. XRCC4 has a mass of approximately 38 kDa. This protein is widely expressed in various tissues with significant levels noted in the nucleus reflecting its direct involvement in DNA repair processes.
Biological function summary
XRCC4 plays a pivotal role in maintaining genetic stability. It is a vital component of the DNA repair machinery and functions as part of a complex with DNA ligase IV. This complex ensures the appropriate joining of DNA ends which is critical for cell survival and prevention of chromosomal aberrations. Without XRCC4 cells would face higher mutational rates and increased genomic instability leading to cellular dysfunction and disease progression.
Pathways
XRCC4 is integral to the non-homologous end joining pathway a major DNA double-strand break repair mechanism in human cells. It interacts with DNA-dependent protein kinase (DNA-PK) and DNA ligase IV during the repair process. Within the NHEJ pathway XRCC4 collaborates closely with DNA ligase IV to seal DNA breaks effectively. It also engages with other proteins such as Ku70/Ku80 to ensure proper coordination of DNA repair activities within the cell.
XRCC4 stands as an important factor in genomic integrity with its dysfunction associating with cancer and immunodeficiency disorders. Mutations or deficiencies in XRCC4 can lead to a higher incidence of leukemia due to the accumulation of genomic instability. Connections between XRCC4 and the ATM protein have been highlighted in research with ATM being another critical player in DNA repair pathways. Defective XRCC4 or its interactions can exacerbate disease progression making it a significant target for therapeutic interventions.