Abcam, ab97440, Anti-ATP6V0A4 antibody

Size: 50µL
Rabbit Polyclonal ATP6V0A4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V0A4 aa 50-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP6V0A4 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.Q9HBG4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V0A4 also known by its alternate name V-ATPase 116 kDa subunit a isoform 4 plays a significant role in acidifying intracellular compartments. This protein is part of the vacuolar ATPase (V-ATPase) complex a proton pump that utilizes ATP hydrolysis to translocate protons across cellular membranes. ATP6V0A4 possesses a mass of approximately 116 kDa and sees expression mainly in the kidney particularly in the intercalated cells of the renal collecting duct. Its expression ensures efficient acid-base homeostasis by aiding the secretion of protons into the urinary space.
Biological function summary
ATP6V0A4 is essential for maintaining proper ion balance and pH regulation in the kidney. It is a component of the V-ATPase complex which comprises multiple subunits working together to pump protons. By participating in intracellular acidification this protein facilitates various processes like protein degradation receptor-mediated endocytosis and coupled transport. The function of the V-ATPase complex in which ATP6V0A4 resides ensures cell survival in acidic environments and supports cellular homeostasis.
Pathways
ATP6V0A4 plays a role in renal acidification and the regulation of systemic blood pH levels. It functions within the context of ion transport pathways in the kidney contributing to the bicarbonate reabsorption process. In these pathways ATP6V0A4 interacts with other components of the V-ATPase complex and associates with proteins like anion exchanger 1 (AE1) to help maintain electrolyte balance and acid-base equilibrium in the body.
Mutations in ATP6V0A4 connect to distal renal tubular acidosis (dRTA) a condition characterized by an inability to effectively acidify urine due to renal tubular dysfunction. This disorder can lead to metabolic acidosis failure to thrive and nephrocalcinosis. Additionally ATP6V0A4 may relate to autosomal recessive inheritance patterns indirectly involving various proteins in acid-base regulation pathways. Understanding these interactions provides potential avenues for therapeutic intervention in dRTA and related conditions.