Abcam, ab97642, Anti-NLK antibody

Size: 25µL / 100µL
Rabbit Polyclonal NLK antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NLK aa 250-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NLK aa 250-500. The exact immunogen used to generate this antibody is proprietary information.Q9UBE8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NLK (Nemo-Like Kinase) functions as a serine/threonine protein kinase involved in various signaling pathways. It alternatively is known as nemo-like protein or nlk. The molecular mass of NLK is approximately 54 kDa. Expression of NLK occurs in various tissues but it is highly observed in the brain and testis. This wide expression suggests that NLK may have diverse functional roles.
Biological function summary
NLK participates in regulating cellular processes such as cell cycle apoptosis and transcription. It is not part of a larger complex but works within signaling pathways to influence these processes. NLK modulates the activity of several transcription factors particularly in the Wnt/beta-catenin signaling pathway where it negatively regulates the signaling to control gene expression relevant to cell proliferation and differentiation.
Pathways
NLK is an important player in the Wnt/beta-catenin and MAPK pathways. In the Wnt/beta-catenin pathway it associates with proteins such as TCF/LEF and beta-catenin acting to modulate transcription by phosphorylating these molecules. The MAPK pathway involves NLK in response to stress signals coordinating with proteins like TAK1 to mediate cellular responses. These pathways illustrate NLK’s role in transmitting signals that govern cell fate and stress responses.
NLK has links to cancer and neurodegenerative diseases. Alterations in NLK expression or function can contribute to the development and progression of cancers by affecting the regulation of cell proliferation and apoptosis. Specifically dysregulation in the Wnt/beta-catenin pathway in which NLK acts relates to colorectal cancers. In the context of neurodegenerative diseases NLK has connections with the protein TDP-43 which is implicated in diseases such as amyotrophic lateral sclerosis highlighting its role in neuronal health and potential links to neuronal degradation.